Highly pathogenic avian influenza viruses (HPAIVs) of H5 subtype have persistently caused outbreaks in domestic poultry and wild birds worldwide and sporadically infected humans. Rapid and accurate diagnosis is one of the key strategies for the control of H5 HPAIVs. However, the sensitivity of the diagnosis of H5 HPAIVs has gradually reduced due to extensive antigenic variation during their evo...

Abstract H5N1 vaccines adjuvanted in the oil-in-water emulsion MF59 are maintained Strategic National Stockpile for pre-pandemic preparedness. The US population currently naïve to H5 and a prime/boost regimen will likely be needed engender protective response. Ideally, rapid response pandemic influenza would benefit from being able induce protection single dose of vaccine. Here, we have used sy...

The linker histones (H1 or H5) which play a key role in the folding of chromatin, are general repressors of gene expression. Nuclei of the mature chicken erythrocytes (and in some mammalian cells) contain both of them. Although the interaction of H5 with DNA is stronger than that of H1, it does not prevent the transcription of some erythroid-specific genes. It has been shown that some modificat...

BACKGROUND Studies have shown that DNA vaccines can induce protective immunity, which demonstrated the high potential of DNA vaccines as an alternative to inactivated vaccines. Vaccines are frequently formulated with adjuvants to improve their release, delivery and presentation to the host immune system. METHODS The H5 gene of H5N1 virus (A/Ck/Malaysia/5858/04) was cloned separately into pcDN...

A new 3D, spin-state-selective coherence transfer NMR experiment is described that yields accurate measurements for eight scalar or dipolar couplings within a spin system composed of a methylene adjacent to a methine group. Implementations of the experiment have been optimized for proteins and for nucleic acids. The experiments are demonstrated for Cbeta-Calpha moieties of the third IgG-binding...

To obtain stable and constitutive expression of histone H5 at levels comparable to those observed in normal chicken erythrocytes, an avian self-inactivating retroviral vector was used to transfer the H5 gene into cells which do not express this protein. The vector, pDAH5, was obtained by removing the CAAT and TATA boxes of the 3'LTR of the avian leukosis virus RAV-2 and inserting the H5 sequenc...

introduction: in the current study, we compared h5-index provided by google scholar (gs), impact factor (if) provided by web of sciences (wos), and scimago journal rank indicator (sjr) provided by scopus for quality assessment of nuclear medicine journals. methods: 2013 h5-index, 2012 if, and 2011 sjr of nuclear medicine journals were extracted from their publishers namely gs, wos, and scopus....